We studied what's in your retainer. Here's what we found.

ResearchScience2026 / 10 / 01

Written by Andrea H. Westlie, PhD

Chewing simulation chambers used in the retainer wear study

We put clear plastic retainers through six months of simulated chewing, then counted what came off them.

Chewing simulation chambers used in the study. Photo from the study white paper.

If you've ever had braces or aligners, you probably went home with a clear plastic retainer and a simple instruction: wear it every night for life or your teeth will shift.

Many clear retainers are made from PETG, a close cousin of the plastic used in water bottles. If you've worn one for a while, you've probably watched it get scratched, cloudy or cracked. Some people even chew right through theirs. That got us wondering: where does all that plastic go? If you bite down on a piece of plastic every night for years, does it shed tiny pieces of itself? And if so, how many?

So we tested it.

What we did

We made four pairs of partial PETG retainer specimens, each covering three back molars, and fitted them onto model teeth. Then we took them to the Materials Testing Facility at the University at Buffalo School of Dental Medicine and put them in a chewing simulator, a machine built to mimic the motion and force of a human bite.

The machine chewed 58,240 times with about 11 pounds of force, in water kept at body temperature. We used that as a model of roughly six months of nightly wear. We also ran the machine with no retainer in it, so we could tell the difference between particles coming from the retainer and any background contamination.

Afterward, we weighed the retainers and sent the water to independent labs for analysis. One used an electron microscope to look at the particles up close. The other used an infrared technique that counts each particle and confirms what it's made of. Particle counts were available for three tested pairs.

Electron micrograph of PETG particles from the simulated chewing test with particle size measurements
PETG particles from our chewing test, under an electron microscope.

What we found

Every retainer showed visible wear.

Water after the simulated chewing test, with white PETG particles visible
Water from our chewing test, seen through the bottom of the jar. Those white specks are pieces of PETG retainer.

On average, each tested pair shed about 59,000 detectable plastic particles over six months of simulated wear. The roughly 118,000-per-year figure is a linear extrapolation under the same test conditions.

Other measurements from the chewing test
~740 per mL PETG particles in the water after chewing, compared with essentially zero when the machine ran without a retainer.
52 microns The average particle size, about the width of a human hair.
3.4 mg Average plastic lost from each pair of retainers.

And that's likely an undercount. The method we used can only detect particles 15 microns and larger. Anything smaller wasn't counted at all.

Why this matters

For context, one widely cited study estimated annual exposure of 74,000 to 113,000 microplastic particles from the foods it studied and inhalation; it analyzed drinking water separately. Our numbers aren't a direct comparison, since we measured particles released into water in a lab, not particles a person actually swallows. But these numbers suggest that a retainer could be a real source of microplastics that nobody has been counting.

And unlike a water bottle, a retainer isn't something you use for a few minutes. It sits in your mouth for hours, every night, often for years.

What we don't know yet

We want to be clear about the limits of this study. It was a lab simulation, not a study of real people. We used purified water instead of saliva, and the test didn't include food, brushing, cleaning or taking the retainer in and out. Everyone's bite is different, and results varied quite a bit even between our samples. This was also a small study, and we plan to expand it.

Most importantly, we don't yet know how many of these particles are swallowed or what they do in the body. Research on the health effects of microplastics is still early.

Please don't stop wearing your retainer. Retainers keep your teeth from shifting back, and that matters. If you have questions or your retainer looks scuffed or cracked, talk to your orthodontist.

The bigger picture

Retention is standard after orthodontic treatment, and clear plastic retainers are a common choice. We think the materials we put in our mouths every night deserve the same scrutiny we've started giving to the plastics in our food and water.

Next, we're testing more retainers, looking for ways to detect particles smaller than 15 microns, and asking a question that matters just as much as how many particles are released: what happens to them afterward? That question is a big part of why we're developing a retainer made from PHA, a plastic produced by microbes, and we'll share what we learn as we test it.

References

1.Westlie, A. H. Microplastic Release from Orthodontic Retainers During Simulated Wear. moses White Paper, 2026. Read the white paper.
2.Cox, K. D. et al. Human Consumption of Microplastics. Environ. Sci. Technol. 2019. doi.org/10.1021/acs.est.9b01517; 2020 correction
3.Meade, M. J.; Millett, D. Retention Protocols and Use of Vacuum-Formed Retainers among Specialist Orthodontists. J. Orthod. 2013. doi.org/10.1179/1465313313Y.0000000066
4.Quinzi, V. et al. A spectroscopic study on orthodontic aligners: First evidence of secondary microplastic detachment after seven days of artificial saliva exposure. Sci. Total Environ. 2023. doi.org/10.1016/j.scitotenv.2022.161356
5.Barile, C. et al. Experimental Assessment of Damage and Microplastic Release during Cyclic Loading of Clear Aligners. PLoS One 2025. doi.org/10.1371/journal.pone.0318207
6.Warunek, J. et al. Orthodontic Derived Microplastics Impact Macrophage Differentiation and Homeostasis. Prog. Orthod. 2026. doi.org/10.1186/s40510-026-00608-3

Keep learning

Glass water carafe and drinking glass with a steel bottle, wooden board, and linen cloth on a kitchen counter Microplastics are everywhere, but you're not powerless